Use the headings below to learn more about the current ongoing research at the Lewin Lab.
The Lewin Lab is working on several projects to study Feline herpesvirus (FHV), which can cause painful and blinding ocular disease in domestic cats.
Antiviral Use in Shelter Cats:
The lab is receiving funding from the Morris Animal Foundation and Stokes Pharmacy to study the effect of antiviral medications for the treatment of FHV in shelter-housed cats. This study will utilize cutting-edge DNA sequencing technology to study the structure of the virus. For additional information on this exciting study please see the study information page on the Morris Animal Foundation website.
LEFT: Dr. Melanie Mironovich hard at work, RIGHT: A study participant, feeling better after some treatment!
**Update February 2021** Our 2020 Summer Scholar, Mikayla Sanford, presented 'Assessing Potential for Antiviral Resistance in Feline Herpesvirus' at the 2021 LSU Phi Zeta research day. Well done Mikayla!
Genetic Sequencing of Feline Herpes Virus:
We have also been busy working with our collaborator at the LSU Center for Computation and Technology, Dr. Lyndon Coghill, on a genome assessment of FHV isolates to assess if certain regions of DNA are associated with disease severity in naturally-infected cats. To view the results of this study, please visit the Virus Genes website.
New Treatment for Feline Herpes Virus:
The lab has been working on assessing the safety of a new antiviral eye medication for use in cats with Feline Herpes Virus. The results have recently been published in the Journal of Feline Medicine and Surgery. Check it out!
The lab has received funding from a USDA sponsored program to study antiviral treatments for bovine herpesvirus.
Dr. Chris Alling will be leading efforts along with help from virologist Dr. Muzammel Haque to find the best treatment for this virus which causes debilitating eye and lung disease in cattle populations worldwide.
**Update February 2021** Dr. Chris Alling presented 'Assessment of Topical Ophthalmic Cidofovir for Treatment of Bovine Herpesvirus-1 Infection in Cattle Using an Ex Vivo Model' at the 2021 LSU Phi Zeta Day and won 2nd place for the best basic science poster (house officer). Well done Chris!
With our collaborator at Cornell School of Veterinary Medicine, Dr. Eric Ledbetter, we are assessing the genome structure of various isolates of Canine herpesvirus, which causes eye disease in domestic dog populations. By understanding the structure of this virus in more detail, we hope to improve prevention and treatment of dogs with this disease. This project is sponsored by the American Kennel Club Canine Health Foundation. http://www.akcchf.org/
**Update December 2020: The results of this exciting study have just been published in the Viruses journal. This open-access paper can be found here!
The group has recently been working with Angela Yoon (LSU DVM candidate) to assess the use of different tests for dry eye in dogs in a variety of environmental conditions. The results will allow for improved interpretation of the test in this species. Please check out the Veterinary Ophthalmology website to read the paper!
**Update October 2020: We are pleased to announce that based on Angela's initial work, another paper has just been accepted by Veterinary Ophthalmology, demonstrating the differences between strip types obtained from different manufacturers. The paper is available on the Veterinary Ophthalmology journal website.
Angela Yoon presenting the results of her research at Tufts University, Aug 2019
Dogs which are scheduled to have cataract surgery at LSU School of Veterinary Medicine are currently being enrolled in a trial to assess the use of a medication to prevent high pressure after surgery. If you are interested in hearing more about this study, please contact the ophthalmology team at LSU SVM directly by email at firstname.lastname@example.org and provide your contact information.
With funding support from the USDA, the lab is working on the collection of ocular microbiome data from cattle with 'Pink Eye' (infectious bovine keratoconjunctivitis) and 'Cancer Eye' (ocular squamous cell carcinoma). These diseases are very economically important to livestock producers and the development of novel therapeutics is a top priority. Using this microbiome data we hope to gain novel insights into these diseases which will direct the development of new treatments.
The members of the lab have recently completed data collection for a project assessing the use of two instruments to measure eye pressure in horses. The data obtained will help ensure consistent measurements are obtained when veterinarians use these instruments. Please visit the Equine Veterinary Journal for more information!
We are also working on a separate project with Dr. Rebekah Joyner (LSU DVM 2020 graduate), assessing the effect of various sedation protocols on eye pressure in horses. This data will allow for accurate interpretation of intraocular pressure in horses with glaucoma and uveitis. The results of this study have recently been published in the Equine Veterinary Journal!
**Update September 2020: Dr. Joyner has presented the results of her study at the annual 2020 American College of Veterinary Ophthalmologists conference. This international event is one of the largest forums for the dissemination of research related to ocular disease in animals. The presentation was well received by the online audience. Well done Dr. Joyner!
The lab is currently involved in a project assessing the use of nanoparticle delivery agents on equine corneal tissue using a novel explant system. Dr. Renee Carter, an ophthalmologist at LSU and leader of this project, is working with the lab to determine the suitability of these new nanoparticles for use in eye medications in horses.
Dr. Melanie Mironovich is working on an exciting project to assess the impact of commonly used ocular diagnostic medications on the growth of bacteria isolated from ocular swabs. These medications include the drops used to dilate your pupils at the eye doctor! Using sophisticated techniques, she will determine if these medications have any effect on the quantity or type of bacteria which can be subsequently isolated from treated eyes. These results will be important information for both veterinary and human ophthalmologists!
**Update February 2021** Dr. Melanie Mironovich presented 'In-vivo Antimicrobial Activity of Topical Ocular Diagnostic Medications' at the 2021 LSU Phi Zeta Day. Well done Melanie!
For a complete list of work by Dr. Lewin, please visit his LSU faculty page.
Previous research performed by Dr. Lewin and others at the University of Wisconsin; Madison:
Herpes Virus Genomics
Dr. Lewin has previously published work assessing the genomics and phylogenetics of feline herpesviruses and other varicelloviruses:
Lewin AC, Kolb AW, McLellan GJ, Bentley E, Bernard KA, Newbury SP, Brandt CR. Genomic, recombinational and phylogenetic characterization of global feline herpesvirus 1 isolates. Virology. 2018 May 1;518:385-97.
Kolb AW, Lewin AC, Trane RM, McLellan GJ, Brandt CR. Phylogenetic and recombination analysis of the herpesvirus genus varicellovirus. BMC genomics. 2017 Dec;18(1):887.
Exotic Animal Ophthalmology
Dr. Lewin has previously published work assessing various ocular parameters in exotic animals:
Lewin AC, Hausmann JC, Miller PE. Intraocular pressure and examination findings in three species of central and south American tree frogs (cruziohyla craspedopus, cruziohyla calcarifer, and anotheca spinosa). Journal of Zoo and Wildlife Medicine. 2017 Sep;48(3):688-93.
Lewin AC, Miller PE. Calibration of the TonoVet and Tono‐Pen Vet tonometers in the porcine eye. Veterinary ophthalmology. 2017 Nov;20(6):571-3.
Snyder KC, Lewin AC, Mans C, McLellan GJ. Tonometer validation and intraocular pressure reference values in the normal chinchilla (Chinchilla lanigera). Veterinary ophthalmology. 2018 Jan;21(1):4-9.